"Contrary to standard scientific texts, the purple dye called crystal violet, a main ingredient in gram staining, does not actually enter bacterial cells, researchers report April 27 in ACS Chemical Biology. Instead, the dye gets trapped in a tight package of sugar-filled polymers, called peptidoglycan, which envelops bacterial cells. The thickness and integrity of the sweet bacterial armor determines whether crystal violet leaves a cell purple or not. That royal shade, or lack of it, reveals a cell’s type of outer structure."
https://www.sciencenews.org/article/bacteria-staining-method-has-long-been-misexplained
Nothing in textbooks is true! Ahhhh
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